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    • 专利摘要:
    1502320 Bacterial extracts from corynebacteria AGENCE NATIONALE DE VALORISATION DE LA RECHERCHE (AN VAR) 5 May 1975 [6 May 1974] 18754/75 Heading A5B Water soluble extracts containing diaminopimelic acid and having a molecular weight in the range 1000 + 200 to 8000 + 200 are obtained by mild extraction carried out by the grinding and homogenizing in an aqueous medium, of delipidated bacterial residues of corynebacteria cells containing diaminopimelic acid and thence isolating the extracts. The extracts may be purified and separated into their components viz, essentially disaccharide - tetrapeptide, tetrasaccharide-heptapeptide and the dimer, trimer and tetramer of the latter by chromatography or filtration e.g. on a polyacrylamide gel. The water soluble extracts in combination with a diluent are usable as pharmaceutical compositions for promoting resistance to viral or bacterial infection.
    公开号:SU727112A3
    申请号:SU752131288
    申请日:1975-05-05
    公开日:1980-04-05
    发明作者:Жолль Пьер；Миглиор-Самур Даниель
    申请人:Ажанс-Насьональ Де Валоризасьон Де Ля Решерш /Анвар/ (Фирма)；
    IPC主号:
    专利说明:

    by centrifugation at a temperature of 0-6 ° C.
    BoflopacTBOpHjviHe extracts are then obtained in a dry state, for example, by lyophilization from solutions obtained in the previous steps that are not dialyzed through an HVf membrane impermeable for substances with a molecular weight above 1000.
    Water-soluble extracts are purified and separated into their composition: by physicochemical cnoco6a viH, namely by chromatography on adsorbents, such as DEAE-cellulose, or by filtration, for example, through a polyacrylamide gel, for example P10, Biogel Rb or Biogel P4, Prepared The water-soluble crude extract after chromatography on DEAE-cellulose contains mainly tetrasaccharide heptapeptide associated with non-aminated-reducing sugar.
    Water-soluble phage extracts have them / stimulate action. Thus, in guinea pigs, subcutaneous feeding of these extracts at doses higher than or equal to 0.1 mg increases the percentage of anti, tel.
    Water soluble extrusions are introduced into the human or animal body to enhance its resistance to infections of viral or bacterial origin. Since their introduction causes the formation of antibodies suitable for the control of pathogenic organisms, they can be added to vaccines to ensure maximum antibody response.
    Water-soluble extracts can be added to drugs and antibiotics, in amounts higher than 0.1% (by weight). They are used orally, rectally, parenterally, or as aerosols. For an adult, the dose is 10-50 mg per day.
    Example. 50 g of bacterial defatted cells of Corynebacte rium parvum are crushed and homogenized in 250 cm of water containing 0.1 cm of 1РР-40 detergent. After stirring for 48 hours. gfy temperature and centrifugation for 30 minutes at a temperature the top layer is heated to. Ammonium sulphate was added to form a solution with 40% saturation and after 12 hours it was centrifuged for 30 minutes at a temperature, O precipitate was obtained, sulphate a was added to the upper layer, and imi was prepared to produce a solution with 70% saturation. After 12 hours, the solution is centrifuged for 30 minutes and a precipitate Oy is obtained.
    Precipitation, 070 and last received upper layer C710 then
    dialyzed separately with respect to distilled water. Solutions that do not dialyze, lyophilize to obtain 3.175 g of the fraction, 0.750 fraction of O, Q and 1.380 g of the fraction, which forms a water-soluble crude extract.
    Example 2. The CJQ fraction obtained in example 1 is purified by chromatography on a column of DEAE-cellulose (height 92 cm, diameter 2, 5 cm), equilibrated with 0.05 M veronal buffer solution at pH 7, eluted with 0.05 M buffer sodium citrate solution with a pH of 3 and collect fractions of 2.3 cm, weighing from 1 to 500. Fractions 150-220 are combined, they contain a tetrasaccharide, an eptapeptide associated with non-aminated dihydrating ones. After lyophilization, 0, 125 g of a water-soluble extract has the following characteristics:
    Appearance: loose powder of white color.
    Composition:
    a) Amino acids (molecular ratio): alanine
    3, glutamic acid 2, diaminopmelic acid (DAP) 2, asparagiva acid, 2, glktsin 2.
    b) Am p ocaxap
    (molecular ratio): N-acetimuraminic acid 2, N-acetylglucosamine 2,
    Molecular ratio, based on 3 alanine residues in a molecule: 4000–200.
    The content of amino acids is 21 + 4%, and amino sugar g 24.5 + 3%.
    The residue consists of non-aminated reducing sugars, the percentage of the WCT is 9.5 + 1.
    The proposed method allows to obtain water-soluble extracts with high immunostimulating properties.
    权利要求:
    Claims (3)
    [1]
    1. A method of obtaining extracts with an immunostimulating effect from pseudo-diphtheria stick cells by disintegrating them and then isolating the target product, characterized in that, in order to obtain water-soluble extracts with high immunostimulating properties, the cells of pseudo-diphtheria rods are degreased and disintegrated by pre-degreasing extracts and disintegrated by means of extracts that have high immunostimulatory properties. .
    [2]
    2. A method according to claim 1, wherein the homogenization is carried out in the presence of a non-ionic detergent.
     727U2 &
    [3]
    3. The method according to claim 1, distinguishing between - Sources of information g and the fact that the selected target is taken into account in the examination of the product is purified by chromatography on DEAE-cellulose, balanced-1. The journal of microbiology, an epididium buffer with a pH of 7.0 ± 0.1 and eluted bymology and immunology, 1972, No. 11, the target product with a buffer of pH 3.0 + 0.1. S. 39-43.
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    同族专利:
    公开号 | 公开日
    DE2519938A1|1975-11-27|
    FR2269961B1|1978-03-24|
    FR2269961A1|1975-12-05|
    SE7505032L|1975-11-07|
    CS196267B2|1980-03-31|
    JPS50155685A|1975-12-16|
    CH604725A5|1978-09-15|
    CA1037028A|1978-08-22|
    IL47205D0|1975-06-25|
    NL7505246A|1975-11-10|
    GB1502320A|1978-03-01|
    IL47205A|1978-06-15|
    US4013788A|1977-03-22|
    BE828635A|1975-10-30|
    DK194975A|1975-11-07|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    FR2320107B1|1975-08-05|1980-01-11|Anvar|
    FR2321896B1|1975-08-29|1978-08-11|Anvar|
    US4148877A|1975-12-29|1979-04-10|Choay S. A.|Fraction capable of inducing in vivo a resistance to bacterial infections, process for obtaining said fraction from bacteria and drugs containing said fraction|
    FR2374042B1|1976-06-04|1980-04-30|Merieux Inst|
    FR2378855B1|1977-01-27|1979-05-11|Cassenne Lab Sa|
    GB1598457A|1977-03-22|1981-09-23|Glaxo Lab Ltd|Process for the preparation of a fraction containing peptidoglycan material from streptomyces griseus|
    JPS58874B2|1979-08-30|1983-01-08|Meguro Kenkyusho Kk|
    DE3011461C2|1980-03-25|1986-10-30|Dr. Madaus & Co, 5000 Köln|Use of propionibacteria|
    FR2480604B1|1980-04-22|1984-02-17|Pasteur Institut|
    US4479935A|1980-04-22|1984-10-30|Institut Pasteur|Fractions extracted from aerobic bacteria, endowed with antitumoral, antibacterial and interferon inducing properties, and process for their preparation|
    FR2490496B1|1980-09-19|1983-05-27|Roussel Uclaf|
    FR2490495B1|1980-09-19|1983-06-24|Roussel Uclaf|
    JPS57163835U|1981-04-07|1982-10-15|
    JPH0742310B2|1984-01-23|1995-05-10|味の素株式会社|Crystallization method of L-aspartyl-L-phenylalanine or its lower alkyl ester|
    IT1237903B|1989-12-14|1993-06-18|Medidom Lab|SEMI-SYNTHETIC DERIVATIVES WITH IMMUNOMODULATING ACTIVITIES FOR PARENTERAL AND ORAL ADMINITRATION|
    EP0681479B1|1993-01-29|1999-04-28|Vetrepharm, Inc.|Immunotherapeutic composition|
    US5976580A|1995-06-07|1999-11-02|Novus International, Inc.|Nutrient formulation and process for enhancing the health, livability, cumulative weight gain or feed efficiency in poultry and other animals|
    JP4759182B2|2001-08-03|2011-08-31|花王株式会社|Water-soluble ginger extract|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    FR7415570A|FR2269961B1|1974-05-06|1974-05-06|
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